Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
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Abbott Msp Liquid Handler W/ Mrt PCR | eBay
Review summary M Correa Posted on Dec 08 at 7: Four different sample extraction volumes 0. These two assays share agbott common features 1 Additional reduction in the lower limits of detection LOD of HIV-1 RNA from the earlier version; 2 New primers and probes designs for recognition of the different viral subtypes and circulating recombinant forms CRFs ; and 3 Reduction in hand-on time by configuring the test near to full automation.
The general principle for NA extraction is similar to that of the Roche method, i. Abbott Molecular is committed to molecular diagnostics.
m2000 REALTIME SYSTEM
It should be mentioned that the genetic diversity of HIV-1 subtypes could present a significant challenge in the accurate VL monitoring of HIV-infected patients who carry non-B subtypes Damond et al. The results abbotr as. Other assay performance characteristics such as analytical specificity, linearity, reproducibility, and precision were not evaluated.
The plot to the left represents the distribution of the VL from the Abbott method assay and the plot to the right represents the distribution of VL from the Roche assay. Consequently, even though both methods are based on the same principle, due to the differences in their proprietary primer and probe designs, compatibility of these two methods has not been well documented.
The Version 2 of the m open mode 0. Dual-labeled fluorescent probes for HIV-1 and HIV-1 QS-specific oligonucleotides were used, each labeled with different reporter dyes and a quencher dye.
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mrealtime-system – hospicare
Using the TaqMan analyzer, the sample extraction volume protocol is 0. Various intrinsic factors including the gene target, Abbott, input volume and different precision of the methods and variations in HIV-1 subtypes could potentially explain some of the discordant results.
The fluorescence activity was converted into VL yields by the analyzer Roche, Together, these data suggest that neither method is better than the other for measuring VL. The difference between these two methods was Log10 0. Furthermore, the input plasma volume is different between the two methods.
Home Publications Conferences Register Contact. Consistently, comparison of the distribution and median values of VL between these two methods also showed similar median VL values but a slight wider distribution by the Roche method than the Abbott method was seen Figure 2. Differences in gene target, test sensitivity, input volume and their abilities to detect different HIV-1 subtypes could potentially explain some of the discordance.
HIV viral particles were first lysed and an HIV-1 Quantitation Standard QS Armored RNA molecule was added to each sample, which was followed by a series of processes which involves abbbott, introduction of magnetic particles, washing to remove unbound particles and abott of nucleic acid NA at elevated temperatures.
The Abbott mrt performed the automated m2000wp and detection process. All VL values were first transformed into the log10 format.
Comparison of HIV-1 Viral Load between Abbott m2000 and Roche COBAS TaqMan Methods
Be the first to review this product. The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al. Detection by real-time technology used two probes of different lengths: The vertical axis represenst the VL differences the Abbott method minus the Roche method mm2000sp the horizontal axis represenst the means of VL.
For examples, the Abbott method has been shown to have better detectability of non-B subtypes Geelen et al. The equation of the fi tted line and the Pearson coeffi cient of determination are presented on the plot.
This method has now become a standard method for monitoring HIV-infected patients on antiretroviral therapy in the United States. Secure We only pass your details on to trusted suppliers at your request. However, this factor should not be the major contributing factor because a recent surveillance study at the University of Maryland Medical Center showed that about This automation enables laboratorians to interface where their skills best contribute to results while minimizing errors and contamination.